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Modified Johnsons J/1
(Borowitska 1988)
This medium is used for Dunaliella salina.
To 980 mL distilled water add
| NaCl | as needed to the desired salinity* |
| MgCl2•6H2O | 1.5 g |
| MgSO4•7H2O | 0.5 g |
| KCl | 0.2 g |
| CaCl2•2H2O | 0.2 g |
| KNO3 | 1.0 g |
| NaHCO3 | 0.043 g |
| KH2PO4 | 0.035 g |
| Fe solution | 10 mL |
| Trace element solution | 10 mL |
*3M approximately equal to 160 PSU
Adjust pH to ca. 7.5 and autoclave
Fe solution
| Na2EDTA | 189 mg |
| FeCl3•6H2O | 244 mg |
| dH2O | to 1000 mL |
Trace element solution
| H3BO3 | 61.0 mg |
| (NH4)6Mo7O24•4H2O | 38.0 mg |
| CuSO4•5H2O | 6.0 mg |
| CoCl2•6H2O | 5.1 mg |
| ZnCl2 | 4.1 mg |
| MnCl2•4H2O | 4.1 mg |
| dH2O | to 1000 mL |
References
Borowitska, M.A. 1988. Algal growth media and sources of algal cultures.
In: Micro-Algal biotechnology (Borowitska, M.A. & Borowitska, L.J. eds) pp.456-465.
Cambridge University Press, New York.
