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Modified Johnsons J/1

(Borowitska 1988)

This medium is used for Dunaliella salina.

To 980 mL distilled water add

NaCl as needed to the desired salinity*
MgCl2•6H2O 1.5 g
MgSO4•7H2O 0.5 g
KCl 0.2 g
CaCl2•2H2O 0.2 g
KNO3 1.0 g
NaHCO3 0.043 g
KH2PO4 0.035 g
Fe solution 10 mL
Trace element solution 10 mL

*3M approximately equal to 160 PSU

Adjust pH to ca. 7.5 and autoclave

Fe solution

Na2EDTA 189 mg
FeCl3•6H2O 244 mg
dH2O to 1000 mL

Trace element solution

H3BO3 61.0 mg
(NH4)6Mo7O24•4H2O 38.0 mg
CuSO4•5H2O 6.0 mg
CoCl2•6H2O 5.1 mg
ZnCl2 4.1 mg
MnCl2•4H2O 4.1 mg
dH2O to 1000 mL


Borowitska, M.A. 1988. Algal growth media and sources of algal cultures.
In: Micro-Algal biotechnology (Borowitska, M.A. & Borowitska, L.J. eds) pp.456-465.
Cambridge University Press, New York.