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SC/SP

(Christensen 1982)


Preparation of SC
Place "a pinch" of CaCO3 at the bottom of a culture tube.
Fill some soil (unfertilized garden soil is suitable) in the culture tube (up to ca. 1 cm from the bottom).
Add water to fill the tube 2/3.
Close the tube with a cotton plug.
The glasses with medium are treated with 100°C steam for 1 hour in an autoclave which is not totally closed.
Repeat treatment after 24h.

Preparation of SP
Place "a pinch" of CaCO3 at the bottom of a culture tube.
Add 1/8 yellow pea
Fill some soil (unfertilized garden soil is suitable) in the culture tube (up to ca. 1 cm from the bottom).
Add water to fill the tube 2/3.
Close the tube with a cotton plug.
The tubes with medium are treated with 100°C steam for 1 hour in an autoclave which is not totally closed.
Repeat treatment after 24h.

Reference
Christensen, T. 1982. Alger i Naturen og i Laboratoriet. 136 pp. 1. ed. Nucleus. (In Danish).